Coding

Part:BBa_K3113010:Design

Designed by: Theresa Keil   Group: iGEM19_Munich   (2019-09-04)


MCP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N55K to get a high affinity mutant. V75E and A81G to prevent capsid assembly. T3A to remove illegal restriction site.

Source

This sequence was given to the team by PhD student Christoph Gruber. The sequence originates in the Bacteriophage MS2.


References